Effects of Extremely Low Frequency Magnetic Fields on Blood Coagulation in Mice: An Initial Study

OF1 mice were chronically exposed to a 50-Hz sinusoidal East–West magnetic field 15 mT (rms), in order to evaluate the blood coagulation variations related to the effect of this nonionizing radiation. Mating and pregnancy of ancestors (first generation), and birth, lactation, and development of second-generation female mice until adulthood took place in the experimental field. A global blood coagulation study of both control and exposed 14to 15-week-old and 50to 52-week-old, secondgeneration females was carried out. Plasma calcium content was determined by atomic absorption spectrophotometry. Different steps of blood coagulation were studied by thromboelastography (TEG) in whole blood (WB), platelet-rich plasma (PRP), and platelet-poor plasma (PPP). A significant decrease (approximately 34.5%) of calcium *Correspondence: Dolores Vallejo, Departamento de Fisiologı́a, Edificio de Ciencias, Universidad de Alcalá, E-28871 Alcalá de Henares, Madrid, Spain; Fax: 34-91-8855080; E-mail: dolores.